Alabama Neuroscience Blueprint Core Center

 

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Core D - Neuroimaging

The Neuroimaging Core expands currently available imaging modalities at UAB and dramatically enhances the ability to visualize neuronal structure and function in living experimental animals. The goals for Core D include:

Assist investigators to effectively use the state-of-the-art imaging equipment in the core. This service includes educating and training users, and maintaining the equipment ready for users's experiments. We currently provide:

  • A multiphoton laser scanning fluorescence microscope (Prairie Technologies Ultima IV). This system is dedicated to in vivo brain imaging through thinned skull or craniotomy, as well as for in vitro imaging of tissue slices (e.g. acute/cultured brain slices).
  • An optical fiber based laser scanning confocal system (Leica FCM 1000) is available to allow cellular imaging at depths not reachable by multiphoton through the skull or cranial window.
  • Image processing and analysis. Two high-performance workstations loaded with Imaris full version (Bitplane) and Neurolucida (MicroBrightField, Inc.) are installed to meet the most sophisticated imaging analysis needs.

Participate actively in the projects. This may include every step leading to a complete imaging project, for instance, initial feasibility studies, experimental design, image acquisition and analysis, and reading grant applications and drafts of papers.

Collaborate in developing novel imaging methods to enhance the imaging capability of the core and to find the technical solutions to the problems from the neuroscience research. For example, second harmonic, fluorescence lifetime and transient absorption can provide additional molecular contrasts in imaging. Those capabilities can be built readily by the available components on an existing microscope main frame.

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Neuroscience Core Day Presentation

Note: When appropriate, please acknowledge the use of the Alabama Neuroscience Blueprint Core Center: "This work was supported by NIH Neuroscience Blueprint Core Grant NS057098 to the University of Alabama at Birmingham."

  Core D Pic

                Director:         Kent Keyser, Ph.D.

          Co-Directors:         Lucas Pozzo-Miller, Ph.D.

                                     Kurt Zinn, Ph.D.

 

 

Contact Information:

 

Tong Ye, Ph.D., Technical Director

Assistant Professor

Department of Neurobiology, SHEL-913
University of Alabama at Birmingham
1825 University Blvd.
Birmingham, AL 35294-2182
Phone: (205) 975-5626
Fax: (205) 975-7394
E-mail: tye@nrc.uab.edu